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Author Guidelines

Life Science International Journal Quarterly  peer-reviewed open access scientific journal. LSIJ dedicated to the latest advancement of all theoretical and scientific aspects of Medical Sciences, Biological Sciences, Molecular biology, Biotechnology. The objectives of the journal are to promote and publish original high-quality research and to provide a forum to researchers and industry practitioners for exchanging ideas, knowledge, and experience.

The journal covers a wide range of topics in the field of Life Sciences, including biology, medicine, and agriculture and ecology and much more. Read More

It publishes authoritative reviews, high-quality original research papers,  article series, insight, letter to the editor, highlight, and news and views.

  • Offers high-quality, original results in both basic and applied Life Science research
  • Covers a wide range of topics, including molecular characterization of genes; energized proteins; plant science; microsatellite markers and other new evaluation tools

How to Submit Manuscript

Authors are requested to submit their manuscripts electronically using the Online Manuscript Submission system. This will guide the author stepwise through the submission process. Please submit separate files for a title page, main text, tables, and figures etc. The editorial office will acknowledge receipt of your manuscript. Please give names and complete contact details including the e-mail address of at least three potential reviewers.

Create a user account

Before you can start working with the system, it is necessary to create an author account for you. An account can be created by anyone from website by clicking on “Register” button.

Submit the article

To submit the new article, go to the Article Tab, and click on “Submit new article” button.

Types of Manuscripts

Research Article

These articles should be clearly describing new and carefully confirmed results and experimental procedure which should be given in required details for others to verify the work.

Review Articles

Submission of reviews and perspective covering topics of current interest are welcome and encouraged.

Mini Reviews

Mini-reviews should be concise and no longer than 3 – 4 printed pages (about 8 – 12 manuscript pages) and full-length reviews should be concise and no longer than 6 – 8 printed pages

Short Communication

A short communication is suitable for recording the results of complete small investigations or giving details of new model or hypothesis, innovative methods, techniques or apparatus. The style of main sections need not conform to that of a full length of papers. However, mentioning subtitles like Introduction, Methodology, etc. should be avoided. Short communications are 3 to 4 print pages

Preparation of Manuscripts

The authors have to check the manuscript extensively for grammatical and spelling mistakes before submission. The manuscript should be prepared in English using “MS-word” with 1cm margin on all side (Top, Bottom, Left and Right) of the page. “Times New Roman” font should be used. The font size should be 12 pt, but Title should be 14pt. All animals, plants and microorganism’s scientific names should be written in italic.

Division into sections

 Manuscripts should be divided into the following sections:

  • Title
  • Abstract
  • Keywords
  • Introduction
  • Materials and Methods
  • Results
  • Discussion
  • Conclusions
  • Ethics Statements
  • Author Contributions
  • Acknowledgements
  • References
  • Figure/Table Titles and Legends
  • Conventions and Abbreviations

For detailed instruction for preparing an article is shown below:

1. Title

Titles should be clear, concise, and describe the content of your manuscript. Abbreviations that are very unique, technical, and only understood by a small number of experts should be avoided if possible. The total length of the title should not exceed 150 characters (including spaces). Also, note that title should be written in sentence case (in sentence case, the first letter of the first word and the first letter of any proper noun are capital letters).

2. Abstract

The second page of the manuscript should contain the abstract only (250 words only). For research articles it should be structured as follows:

  • Scope
  • Methods and results
  • Conclusion

3. Keywords

Keywords should identify the topic, academic subdiscipline, methodology and anything that describes the research. These words separated by semicolon (;)

4. Introduction

The introduction should briefly describe the research’s background without subheadings. The citation should be conducted as appropriate when writing a review for your researching area.

5. Materials and Methods

In this section, information necessary to reproduce the experiment or survey conducted in the research should be organized and described. Provide the name of the manufacturer and model number of reagents and equipment if you use them. Methods such as protocols which have been well established should be quoted appropriately, and all modifications should be stated if altered.
Statistical method and software should be stated. A study designed to use animals and/or human subjects need to declare ethics statements.

6. Results

Results should be described as accurate as possible. Figures and tables may be used. It should be divided into subsections with appropriate subheadings without any numbering. Experimental maneuver should be stated in “Materials and Methods” section, and duplication in “Results” should be avoided.

7. Discussion

Make discussion verify your hypothesis by indicating results and quoting related works. Discussion should explain the importance of the result and present future prospects. If you choose “Results and Discussion” for section title, result and speculation should be explicitly expressed.

8. Conclusions

Briefly state the work’s outcome, what the finding is and how important it should be in academic contexts. Authors should carefully avoid overstating.

9. Ethics Statements

In any research that includes human participants, authors should state how they ensure the quality and integrity of your research; how they got informed consent; how they respect the confidentiality and anonymity regarding the trial subject and how they will avoid harm to the subjects. Whether the subjects participated in the study voluntarily should be stated as well.
Authors that include animals should obtain appropriate approval from their Institutional review board, ethics committee, or equivalent committees, and should state it.
LSIJ expects authors of human or animal researches to include Ethics statements at an end of the manuscript .

10. Author Contributions

Please describe each author’s contribution in a list, bearing in mind the authorship policy In the submission form, the following five contributions will be asked for each author. At least one contribution from each of 1) and 2) is required to be identified as an author.

1) Conceived and designed the work

  • Acquired the data
  • Analyzed and/or interpreted the data


  • Drafted the work
  • Revised and approved the work

11. Acknowledgements

Express your gratitude toward all parties related to your research. However, acknowledgments to your funding organization should be included in “Funding” section. It is recommended to obtain approval from all organizations and individuals appearing in this section about mentioning.

12. References

References should be numbered sequentially in the order in which they are cited in the text. The numbers should be set in brackets, thus [2, 18]. References are to be collected in numerical order at the end of the manuscript under the heading "References"; they should also be typed with double spacing throughout. Papers with multiple authors should be limited to listing five authors. Where there are more than five authors, the first four should be listed, followed by et al. Please include the title of the manuscript in full, followed by a full stop. Journal names should be abbreviated according to the practice of PubMed. The abbreviated journal name and the volume number should be in italics. Please note the following examples.


[1].  Keppler K., Hein E.-M., Humpf H.-U. Metabolism of quercetin and rutin by the pig caecal microflora prepared by freeze-preservation. Mol. Nutr. Food Res. 2006, 50, 686-695.


[2].  Eisenbrand G., Dayan A. D., Elias P. S., Grunow W., Schlatter, J. (Eds.). Carcinogenic and Anticarcinogenic Factors in Food, Wiley-VCH Verlag, Weinheim 2003.

Chapter in a book:

[3].  Geis A., in: Heller, K. J. (Ed.), Genetically Engineered Food - Methods and Detection, Wiley-VCH Verlag, Weinheim 2003, pp. 100-118.

Conflict of interest statement

Authors are responsible for disclosing all and any financial and personal relationships with themselves and others that might bias their work. To prevent ambiguity, authors must state explicitly whether potential conflicts do or do not exist. Should such a conflict of interest exist, a statement to that effect must be included in a separate paragraph following on from the acknowledgments section detailing - for each author - the nature of the conflict. Even if there is none, this should also be stated. This is a mandatory requirement for all articles.


Tables with suitable captions at the top and numbered with Arabic numerals should be collected at the end of the text on separate sheets (one page per table). Column headings should be kept as brief as possible and indicate units (in parentheses). Footnotes to tables should be indicated with a), b), c) etc. and typed on the same page as the table.


Figures  with suitable captions at the top and numbered with Arabic numerals should be collected at the end of the text. Please provide title and a brief description or only title so that readers can comprehend the contents of the paper without perusing it. When some separate images are combined in one figure, put subtitles beginning with “(a),” “(b),” and so on to each of them. Symbols and error bars must be defined in the legend.

Structural formulae

Structural formulae should be drawn in the manuscript in the position where they belong. They must be numbered in consecutive order with the other figures.


Mathematical and chemical equations are to be written in the manuscript at the place in which they belong and should be marked by Arabic numerals in parentheses in the right margin in the order of their appearance.


Abbreviations are hindrances to a reader working in a field other than that of the author, and to abstractors. Therefore, their use should be restricted to a minimum. Abbreviations should be introduced only when repeatedly used. Abbreviations used only in a table or a figure may be defined in the legend. Standard abbreviations may be used in the title and keywords. If nonstandard abbreviations are used in the Abstract they should be defined in the Abstract, in the list of abbreviations of the manuscript, as well as when first used in the body of the paper.

Standard abbreviations

The abbreviations as listed below may be used without definition in the articles published in MNF. Please refer to Section 5.14 for the correct usage of abbreviations in MNF.


ACN    acetonitrile

A/D      analog to digital converter

amu     atomic mass unit

API      atmospheric pressure ionization

BMI     body mass index

bp        base pairs

BSA     bovine serum albumin

CBB     Coomassie Brilliant Blue

CE       capillary electrophoresis

CEC     capillary electrochromatography

CFE     continuous flowelectrophoresis

CID      collision-induced dissociation

cpm     counts per minute

CV       coefficient of variation

CZE     capillary zone electrophoresis

1-D      one-dimensional

2-D      two-dimensional

Da        dalton (molecular mass)

DAD    diode-array detection (or diodearray detector)

2-DE    two-dimensional gel electrophoresis

DMEM Dulbecco's modified Eagle medium

DMF    N,N-dimethylformamide

DMSO dimethyl sulfoxide

dsDNA double-stranded DNA

DTT     dithiothreitol

EDTA  ethylenediaminetetraacetic acid

EGTA  ethylene glycol-bis (β-aminoethylether)-N,N,N',N'-tetraacetic acid

ELISA  enzyme-linked immunosorbent assay

EOF     electroosmotic flow

ER       endoplasmic reticulum

ESI       electrospray ionization

FAB     fast atomic bombardment

FAME  fatty acid methyl esters

FITC    fluorescein isothiocyanate

GC       gas chromatography

GMO   genetically modified organism

HDL    high density lipoprotein

HEPES N-(2-hydroxyethyl)piperazine-2'-(2-ethane-sulfonic acid)

HPCE   high-performance capillary electrophoresis

HPLC   high-performance liquid chromatography

HSA     human serum albumin

HTML  hypertext mark-up language

id         inside diameter

IEF       isoelectric focusing

Ig         immunoglobulin

IL         interleukin

IFN      interferon

IT         ion trap

kbp      kilobase pairs

kDa      kilodalton (molecular mass)

LC       liquid chromatography

LDL     low denisty lipoprotein

LOD    limit of detection

LOQ    limit of quantitation

LPS      lipopolysaccharide

mAb    monoclonal antibody

MALDI-MS     matrix-assisted laser desorption/ionization mass spectrometry

Mbp     megabase pairs

MHC    major histocompatibility complex

MOPS  3-(N-morpholino)propanesulfonic acid

Mr        relative molecular mass (dimensionless)

MS       mass spectrometry

MS/MS tandem mass spectrometry

MUFA monounsaturated fatty acid

m/z      mass-to-charge ratio

NMR    nuclear magnetic resonance

od        outside diameter

OD       optical density

ORF     open reading frame

PAGE  polyacrylamide gel electrophoresis

PBS      phosphate-buffered saline

PCR     polymerase chain reaction

PEG     polyethylene glycol

pI         isoelectric point

PMSF   phenylmethylsulfonyl fluoride

PMT     photomultiplier tube

ppm     parts per million

PTFE    polytetrafluoroethylene

PUFA   polyunsaturated fatty acid

PVP     polyvinylpyrrolidone

RIA      radioimmunoassay

RNA    ribonucleic acid

RP        reversed phase

rpm      rotations per minute

RSD     relative standard deviation

RT-PCR           reverse transcriptase-PCR

SCFA   short chain fatty acid

SD       standard deviation

SDS     sodium dodecyl sulfate

SEM     standard error of the mean

SIM      selected ion monitoring

S/N      signal-to-noise ratio

SPE      solid-phase extraction

ssDNA single-stranded DNA

TFA     trifluoroacetic acid

THF     tetrahydrofuran

TIC      total ion current

TLC     thin-layer chromatography

TOF     time of flight

Tris      tris(hydroxymethyl)aminomethane

URL     uniform resource locator

Vh       volt x hours

VLDL  very low-density lipoprotein


Plagiarism Policy 

There is also Plagiarism Policy in LSIJ. The submitted manuscript must have less than 25% Plagiarism 


Submission Preparation Checklist

As part of the submission process, authors are required to check off their submission's compliance with all of the following items, and submissions may be returned to authors that do not adhere to these guidelines.

  1. The submission has not been previously published, nor is it before another journal for consideration (or an explanation has been provided in Comments to the Editor).
  2. The submission file is in OpenOffice, Microsoft Word, RTF, or WordPerfect document file format.
  3. Where available, URLs for the references have been provided.
  4. The text is single-spaced; uses a 12-point font; employs italics, rather than underlining (except with URL addresses); and all illustrations, figures, and tables are placed within the text at the appropriate points, rather than at the end.
  5. The text adheres to the stylistic and bibliographic requirements outlined in the Author Guidelines, which is found in About the Journal.
  6. If submitting to a peer-reviewed section of the journal, the instructions in Ensuring a Blind Review have been followed.

Copyright Notice

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).


Privacy Statement

The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.